Journal: Frontiers in Molecular Neuroscience

Article Title: TIMP-1 Attenuates the Development of Inflammatory Pain Through MMP-Dependent and Receptor-Mediated Cell Signaling Mechanisms

doi: 10.3389/fnmol.2019.00220

Figure Lengend Snippet: Assessing TIMP-1 expression along peripheral nociceptive circuit following cutaneous inflammation. (A) Cutaneous inflammation does not alter overall TIMP-1 protein expression in lumbar spinal cord or (B) DRG, but does increase protein expression in panel (C) hairy skin. n = 4/condition, ∗ indicate significant differences compared to naïve controls, p < 0.05, and error bars depict SEM.

Article Snippet: WT and T1KO mice received injections (s.c.) of recombinant murine (rm)TIMP-1 (10 ng/μL, 10 μL; R&D Systems; Minneapolis, MN) immediately following CFA injection (10 μL) into the right hindpaw.

Techniques: Expressing

Journal: Frontiers in Molecular Neuroscience

Article Title: TIMP-1 Attenuates the Development of Inflammatory Pain Through MMP-Dependent and Receptor-Mediated Cell Signaling Mechanisms

doi: 10.3389/fnmol.2019.00220

Figure Lengend Snippet: Cellular colocalization of TIMP-1 expression. (A) Immunostaining (20×) of naïve and inflamed lumbar spinal cord 24 h following inflammation. TIMP-1 (green) expression is localized to GFAP-positive astrocytes (red). n = 3/condition, scale bar 20 μm. (B) Immunostaining (20×) of naïve and inflamed lumbar DRG 24 h following inflammation. TIMP-1 (green) expression is colocalized with by GFAP-positive satellite glial cells (red). n = 3/condition, scale bar 20 μm. (C) Immunostaining (40×) of hindpaw hairy skin shows K14-positive keratinocytes (red) upregulate TIMP-1 (green) 24 h following inflammation compared to naïve control. n = 3/condition, scale bar 50 μm.

Article Snippet: WT and T1KO mice received injections (s.c.) of recombinant murine (rm)TIMP-1 (10 ng/μL, 10 μL; R&D Systems; Minneapolis, MN) immediately following CFA injection (10 μL) into the right hindpaw.

Techniques: Expressing, Immunostaining

Journal: Frontiers in Molecular Neuroscience

Article Title: TIMP-1 Attenuates the Development of Inflammatory Pain Through MMP-Dependent and Receptor-Mediated Cell Signaling Mechanisms

doi: 10.3389/fnmol.2019.00220

Figure Lengend Snippet: Mice lacking TIMP-1 develop thermal and mechanical hypersensitivity following cutaneous inflammation. (A) No differences in baseline thermal PWL are exhibited between T1KO and WT mice ( n = 16/condition). (B) Inflamed T1KO mice exhibit significantly reduced PWLs compared to inflamed WT mice and naïve WT and T1KO mice ( n = 8/condition). (C) Baseline assessment of mechanical PWTs revealed no genotypic differences between T1KO ( n = 20) and WT mice ( n = 18). (D) T1KO mice develop significantly reduced PWTs 1, 5, and 7 days following CFA administration compared to WT controls ( n = 8–10/condition). ∗ = signficantly different from mice in all other conditions, p < 0.05, and error bars depict SEM.

Article Snippet: WT and T1KO mice received injections (s.c.) of recombinant murine (rm)TIMP-1 (10 ng/μL, 10 μL; R&D Systems; Minneapolis, MN) immediately following CFA injection (10 μL) into the right hindpaw.

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: TIMP-1 Attenuates the Development of Inflammatory Pain Through MMP-Dependent and Receptor-Mediated Cell Signaling Mechanisms

doi: 10.3389/fnmol.2019.00220

Figure Lengend Snippet: Mice lacking TIMP-1 show increased sensitivity in non-inflamed tissues. (A) Injection of CFA into the hairy skin causes mechanical hypersensitivity on the plantar surface of the paw to develop 1 day following inflammation in T1KO, but not WT, mice. (B) Graph depicting mechanical responsiveness following inflammation collapsed across time. Inflamed T1KO mice greater mechanical sensitivity overall following cutaneous inflammation. (C) Administration of TIMP-1(FL), TIMP-1(N), or TIMP-1(C) into the hairy skin at the time of inflammation prevents the development of mechanical hypersensitivity in T1KO mice. (D) Hindpaw administration of CFA produces mechanical hypersensitivity on the paw contralateral to inflammation in T1KO relative txo WT mice. Treatment with rmTIMP-1 attenuated contralateral hypersensitivity in T1KO mice. PWT are presented as change from baseline. n = 8/condition, ∗ represent significant differences relative to naïve controls, p < 0.05, and error bars depict SEM. # significantly different from WT mice.

Article Snippet: WT and T1KO mice received injections (s.c.) of recombinant murine (rm)TIMP-1 (10 ng/μL, 10 μL; R&D Systems; Minneapolis, MN) immediately following CFA injection (10 μL) into the right hindpaw.

Techniques: Injection

Journal: Frontiers in Molecular Neuroscience

Article Title: TIMP-1 Attenuates the Development of Inflammatory Pain Through MMP-Dependent and Receptor-Mediated Cell Signaling Mechanisms

doi: 10.3389/fnmol.2019.00220

Figure Lengend Snippet: Replacement of TIMP-1 attenuates ongoing inflammatory pain in WT mice. Comparison of pre-conditioning and post-conditioning time spent in the clonidine paired chamber show a significant increase in the post-conditioning time in the Vehicle/CFA treated mice, but not the rTIMP-1/CFA treated mice. ∗ p < 0.01 vs. pre-conditioning time. Sample size CFA/rTIMP-1 = 9; CFA/Veh = 8. Error bars = S.E.M.

Article Snippet: WT and T1KO mice received injections (s.c.) of recombinant murine (rm)TIMP-1 (10 ng/μL, 10 μL; R&D Systems; Minneapolis, MN) immediately following CFA injection (10 μL) into the right hindpaw.

Techniques: Comparison